Secreted Bacterial Outer Membrane Vesicles (OMVs) and their role in pathogenesis

Abstract

OMVs are spherical extracellular secretions for proteins and lipids of gram-negative bacteria with Variety of functional roles of OMVs important for baceria growth survival and proliferation. OmpU in Vibrio cholerae confers a lot of properties like Bile salt resistance ,Resistance against anti-microbial peptides and in Survival and colonization in the gut . The Objective was to Characterize the role of secreted bacterial OMVs in pathogenesis . It was found that In cells treated with rOmpU , rOmpU gets translocated to the mitochondria but gets degraded 4 hours post treatment.But vesicle gets translocated to the mitochondria of the host cell and OmpU is seen to be present intact uptil 24 hrs post-treatment . hence it is Hypothesized that Intact vesicle gets translocated to the mitochondria of the host cell and OmpU gets prevented from degradation by the protective vesicle environment .