The Role of PRC1 and PRC2 in Zebrafish Fin Regeneration

Abstract

In the realm of regenerative capabilities, humans exhibit limited ability, whereas lower vertebrates such as zebrafish and axolotls possess remarkable regenerative powers, being able to regenerate not only their fins and appendages but also internal body organs like the heart, kidney, spine, and brain. This striking contrast in regenerative potential has sparked immense interest in understanding the mechanisms underlying regeneration in lower verte- brates, with the aspiration of unlocking possibilities for human regenerative medicine. The significance of this field of research lies in the prospect of comprehending regener- ation processes and subsequently applying this knowledge to facilitate regenerative thera- pies in humans. If we can uncover the intricacies of regeneration in lower vertebrates like zebrafish, it may pave the way for innovative approaches to regenerating damaged or lost tissues in humans, addressing various medical challenges. So as a small contribution to this field of research, in this study, we focus on exploring the role of Polycomb group (PcG) proteins, specifically PRC1 and PRC2, as vital epige- netic factors contributing to regeneration and related cellular processes and pathways. By investigating their involvement, we aim to shed light on the underlying molecular mecha- nisms that drive the regenerative potential in zebrafish. This research involves the use of specific inhibitors targeting PRC1 (PRT4165) and PRC2 (GSK343) expression, which will allow us to understand their precise roles and functions in the process of fin regeneration. The outcomes of this research are anticipated to hold significant implications for regen- erative biology. By unraveling the contributions of PRC1 and PRC2 in zebrafish fin regen- eration, the goal is to to broaden our knowledge of epigenetic regulation and its impact on regenerative processes, bringing us closer to a future where tissue repair and regeneration become a reality.