Single cell-level detection and quantitation of leaky protein expression from any strongly regulated bacterial system
| dc.contributor.author | Arora, Kanika | |
| dc.contributor.author | Guptasarma, P. | |
| dc.date.accessioned | 2020-12-09T05:28:20Z | |
| dc.date.available | 2020-12-09T05:28:20Z | |
| dc.date.issued | 2015 | |
| dc.description | Only IISERM authors are available in the record. | |
| dc.description.abstract | Extremely low levels of "leaky" expression of genes in bacterial protein expression systems can severely curtail cell viability when expressed proteins are toxic. A general method for sensitive detection of such expression is lacking. Here, we present a method based on microscopic visualization of a fluorescent "reporter" protein (RFP-HU-A) constructed by fusing red fluorescent protein (RFP) to the N-terminus of a nucleoid-associated, histone-like DNA-binding protein, HU-A. Localization of RFP-HU-A within nucleoids facilitates detection, quantitation, and characterization of leaky expression at the single-cell level. | en_US |
| dc.identifier.citation | Analytical Biochemistry, 484 | en_US |
| dc.identifier.other | 10.1016/j.ab.2015.06.011 | |
| dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0003269715003048 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/2857 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Science Direct | en_US |
| dc.subject | cell-level | en_US |
| dc.subject | quantitation | en_US |
| dc.subject | protein | en_US |
| dc.subject | bacterial | en_US |
| dc.title | Single cell-level detection and quantitation of leaky protein expression from any strongly regulated bacterial system | en_US |
| dc.type | Article | en_US |