Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/2962
Title: | Resolving the molecular mechanism of cadherin catch bond formation |
Authors: | Rakshit, S. |
Keywords: | Cadherin Mechanism Embryogenesis |
Issue Date: | 2014 |
Publisher: | Nature Publishing Group |
Citation: | Nature Communications,5. |
Abstract: | Classical cadherin Ca2+ -dependent cell-cell adhesion proteins play key roles in embryogenesis and in maintaining tissue integrity. Cadherins mediate robust adhesion by binding in multiple conformations. One of these adhesive states, called an X-dimer, forms catch bonds that strengthen and become longer lived in the presence of mechanical force. Here we use single-molecule force-clamp spectroscopy with an atomic force microscope along with molecular dynamics and steered molecular dynamics simulations to resolve the molecular mechanisms underlying catch bond formation and the role of Ca2+ ions in this process. Our data suggest that tensile force bends the cadherin extracellular region such that they form long-lived, force-induced hydrogen bonds that lock X-dimers into tighter contact. When Ca2+ concentration is decreased, fewer de novo hydrogen bonds are formed and catch bond formation is eliminated. |
Description: | Only IISERM authors are available in the record. |
URI: | https://www.nature.com/articles/ncomms4941 http://hdl.handle.net/123456789/2962 |
Appears in Collections: | Research Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
need to add pdf....odt | 8.12 kB | OpenDocument Text | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.