Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/4426
Title: A novel protein-engineered dsDNA-binding protein (HU-Simulacrum) inspired by HU, a nucleoid-associated DNABII protein
Authors: Thakur, Bhishem
Gupta, Archit
Guptasarma, Purnananda
Keywords: protein-engineered
dsDNA-binding
protein (HU-Simulacrum)
HU
Issue Date: 2021
Publisher: Elsevier
Citation: Biochemical and Biophysical Research Communications, 534, 47-52.
Abstract: HU, a DNA-binding protein, has a helical N-terminal region (NTR) of ∼44 residues and a beta strand- and IDR-rich C-terminal region (CTR) of ∼46 residues. CTR binds to DNA through (i) a clasp (two arginine/lysine-rich, IDR-rich beta hairpins that bind to phosphate groups in the minor groove), (ii) a flat surface (comprising four antiparallel beta strands that abut the major groove), and (iii) a charge cluster (two lysine residues upon a short C-terminal helix). HU forms a dimer displaying extensive inter-subunit CTR-CTR contacts. A single-chain simulacrum of these contacts (HU-Simul) incorporating all DNA-binding elements was created by fusing together the CTRs of Escherichia coli HU-A and Thermus thermophilus HU. HU-Simul is monomeric, binds to dsDNA and cruciform DNA, but not to ssDNA.
Description: Only IISER Mohali authors are available in the record.
URI: https://doi.org/10.1016/j.bbrc.2020.11.088
http://hdl.handle.net/123456789/4426
Appears in Collections:Research Articles

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