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http://hdl.handle.net/123456789/4426
Title: | A novel protein-engineered dsDNA-binding protein (HU-Simulacrum) inspired by HU, a nucleoid-associated DNABII protein |
Authors: | Thakur, Bhishem Gupta, Archit Guptasarma, Purnananda |
Keywords: | protein-engineered dsDNA-binding protein (HU-Simulacrum) HU |
Issue Date: | 2021 |
Publisher: | Elsevier |
Citation: | Biochemical and Biophysical Research Communications, 534, 47-52. |
Abstract: | HU, a DNA-binding protein, has a helical N-terminal region (NTR) of ∼44 residues and a beta strand- and IDR-rich C-terminal region (CTR) of ∼46 residues. CTR binds to DNA through (i) a clasp (two arginine/lysine-rich, IDR-rich beta hairpins that bind to phosphate groups in the minor groove), (ii) a flat surface (comprising four antiparallel beta strands that abut the major groove), and (iii) a charge cluster (two lysine residues upon a short C-terminal helix). HU forms a dimer displaying extensive inter-subunit CTR-CTR contacts. A single-chain simulacrum of these contacts (HU-Simul) incorporating all DNA-binding elements was created by fusing together the CTRs of Escherichia coli HU-A and Thermus thermophilus HU. HU-Simul is monomeric, binds to dsDNA and cruciform DNA, but not to ssDNA. |
Description: | Only IISER Mohali authors are available in the record. |
URI: | https://doi.org/10.1016/j.bbrc.2020.11.088 http://hdl.handle.net/123456789/4426 |
Appears in Collections: | Research Articles |
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File | Description | Size | Format | |
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Need To Add…Full Text_PDF..pdf | Only IISER Mohali authors are available in the record. | 15.36 kB | Adobe PDF | View/Open |
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