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DC Field | Value | Language |
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dc.contributor.author | Agarwal, Aishwarya | - |
dc.contributor.author | Arora, Lisha | - |
dc.contributor.author | Rai, Sandeep K. | - |
dc.contributor.author | Avni, Anamika | - |
dc.contributor.author | Mukhopadhyay, Samrat | - |
dc.date.accessioned | 2023-08-10T10:01:16Z | - |
dc.date.available | 2023-08-10T10:01:16Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Nature Communications, 13(1), 1154 | en_US |
dc.identifier.uri | http://hdl.handle.net/123456789/4454 | - |
dc.description | Only IISERM authors are available in the record | en_US |
dc.description.abstract | Biomolecular condensation via liquid-liquid phase separation of proteins and nucleic acids is associated with a range of critical cellular functions and neurodegenerative diseases. Here, we demonstrate that complex coacervation of the prion protein and α-synuclein within narrow stoichiometry results in the formation of highly dynamic, reversible, thermo-responsive liquid droplets via domain-specific electrostatic interactions between the positively-charged intrinsically disordered N-terminal segment of prion and the acidic C-terminal tail of α-synuclein. The addition of RNA to these coacervates yields multiphasic, vesicle-like, hollow condensates. Picosecond time-resolved measurements revealed the presence of transient electrostatic nanoclusters that are stable on the nanosecond timescale and can undergo breaking-and-making of interactions on slower timescales giving rise to a liquid-like behavior in the mesoscopic regime. The liquid-to-solid transition drives a rapid conversion of complex coacervates into heterotypic amyloids. Our results suggest that synergistic prion-α-synuclein interactions within condensates provide mechanistic underpinnings of their physiological role and overlapping neuropathological features | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Springer Nature | en_US |
dc.subject | Spatiotemporal modulations | en_US |
dc.subject | heterotypic condensates of prion and α-synuclein control | en_US |
dc.subject | amyloid conversion | en_US |
dc.title | Spatiotemporal modulations in heterotypic condensates of prion and α-synuclein control phase transitions and amyloid conversion | en_US |
dc.type | Article | en_US |
Appears in Collections: | Research Articles |
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