Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/4464
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dc.contributor.authorAvni, Anamika-
dc.contributor.authorJoshi, Ashish-
dc.contributor.authorMukhopadhyay, Samrat-
dc.contributor.authorPattanashetty, Swastik G.-
dc.contributor.authorWalimbe, Anuja-
dc.date.accessioned2023-08-10T10:45:35Z-
dc.date.available2023-08-10T10:45:35Z-
dc.date.issued2022-
dc.identifier.citationNature Communications, 13(1), 4378.en_US
dc.identifier.urihttps://doi.org/10.1038/s41467-022-32143-0-
dc.identifier.urihttp://hdl.handle.net/123456789/4464-
dc.descriptionOnly IISERM authors are available in the recorden_US
dc.description.abstractBiomolecular condensates formed via liquid-liquid phase separation (LLPS) are involved in a myriad of critical cellular functions and debilitating neurodegenerative diseases. Elucidating the role of intrinsic disorder and conformational heterogeneity of intrinsically disordered proteins/regions (IDPs/IDRs) in these phase-separated membrane-less organelles is crucial to understanding the mechanism of formation and regulation of biomolecular condensates. Here we introduce a unique single-droplet surface-enhanced Raman scattering (SERS) methodology that utilizes surface-engineered, plasmonic, metal nanoparticles to unveil the inner workings of mesoscopic liquid droplets of Fused in Sarcoma (FUS) in the absence and presence of RNA. These highly sensitive measurements offer unprecedented sensitivity to capture the crucial interactions, conformational heterogeneity, and structural distributions within the condensed phase in a droplet-by-droplet manner. Such an ultra-sensitive single-droplet vibrational methodology can serve as a potent tool to decipher the key molecular drivers of biological phase transitions of a wide range of biomolecular condensates involved in physiology and disease.en_US
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.subjectRaman scatteringen_US
dc.subjectliquid-liquid phase separationen_US
dc.subjectSingle-droplet surface-enhanceden_US
dc.titleSingle-droplet surface-enhanced Raman scattering decodes the molecular determinants of liquid-liquid phase separation.en_US
dc.typeArticleen_US
Appears in Collections:Research Articles

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