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Title: | Ultrasensitive Characterization of the Prion Protein by Surface-Enhanced Raman Scattering: Selective Enhancement via Electrostatic Tethering of the Intrinsically Disordered Domain with Functionalized Silver Nanoparticles |
Authors: | Singh, Swapnil Agarwal, Aishwarya Avni, Anamika Mukhopadhyay, Samrat |
Keywords: | Ultrasensitive Characterization Prion Protein Surface-Enhanced |
Issue Date: | 2021 |
Publisher: | ACS Publications |
Citation: | Journal of Physical Chemistry Letters, 12(12), 3187-3194. |
Abstract: | Surface-enhanced Raman scattering (SERS) circumvents the inherent insensitivity of Raman spectroscopy and offers a powerful tool for the ultrasensitive detection and characterization of biomolecules at low concentrations. Here we show that SERS via electrostatic tethering between surface-modified negatively charged silver nanoparticles and highly positively charged intrinsically disordered N-terminal domain of the prion protein allows highly sensitive and reproducible protein detection and characterization at as low as hundreds of nanomolar protein concentrations. These measurements preferentially illuminate a selective part of the protein due to a sharp dependence of the near-field intensity on the distance between the nanoparticle surface and the protein. We also demonstrate that by shortening the length of the disordered tail it is possible to achieve a domain-selective Raman enhancement to study the C-terminal globular domain. Our tether-length-dependent SERS methodology will serve as a potent, noninvasive, and label-free strategy to detect and characterize a wide range of proteins possessing disordered segments. |
Description: | Only IISERM authors are available in the record. |
URI: | https://doi.org/10.1021/acs.jpclett.1c00240 http://hdl.handle.net/123456789/4651 |
Appears in Collections: | Research Articles |
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Need To Add…Full Text_PDF..pdf | Only IISERM authors are available in the record. | 15.36 kB | Adobe PDF | View/Open |
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