Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/829
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dc.contributor.authorSpandana, Boddu S.-
dc.date.accessioned2017-07-18T05:23:02Z-
dc.date.available2017-07-18T05:23:02Z-
dc.date.issued2017-07-17-
dc.identifier.urihttp://hdl.handle.net/123456789/829-
dc.description.abstractAs a model organism, Drosophila Melanogaster, since the beginning of the last cen- tury has been widely used in scientific experiments, ranging from those of genetics, to neural circuits. This project aims to use them in order to study the changes in the ies metabolites over the course of their life cycle. Flies grown to be resistant against the bacteria, Psuedomonas entomophilia were chosen and grown over several generations, and subsequently, infected with a dead strain of the bacteria. The infected ies were then frozen over three stages of their life cycle, viz. young, moderate and old, for further analysis of the metabolites produced as an immune response to the infection, and how they vary across stages, with the help of Nuclear Magnetic Resonance, NMR. NMR spectroscopy is an analytical technique, applied for identification of a sam- ple's constituents, by determining the change in their atoms' nuclei spin under an externally applied magnetic field, which yields a spectra. Considering its functioning, it could be ideal to identify small, light-weighted compounds, like ies' metabolites. For this project, after conducting 1D and 2D experiments, data collected was sub- jected to several multivariate statistical analyses, like PCA, OPLS-DA and ANOVA. These analyses will allow for understanding the fine differences in metabolites across the three stages and identifying significant metabolites produced triggered by the infection.en_US
dc.description.sponsorshipIISER-Men_US
dc.language.isoenen_US
dc.publisherIISER-Men_US
dc.subjectBiologyen_US
dc.subjectPhysicsen_US
dc.subjectDrosophilaen_US
dc.subjectDrosophila melanogasteren_US
dc.subjectImmunityen_US
dc.subjectNMRen_US
dc.subjectNuclear Magnetic Resonanceen_US
dc.subjectMetabolomicsen_US
dc.titleNMR-based Metabolomics Study of Age-related Immune Response of Drosophila melanogasteren_US
dc.typeThesisen_US
dc.guidePrasad, N.G.-
dc.guideDorai, K.-
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